Test Principle

Multiplex asssays are performed  directly in a microtiter plate. Each assay contains a mixture of different microspheres (Bead Mix). Each bead type is defined by an individual fluorescent color tone and carries a specific detection reagent such as oligonucleotide probes, recombinant antigens or specific proteins on its surface.

During  incubation of the bead mix with the patient sample complementary reaction partner (analytes) bind to the microsphreses.

Subsequently, in a second incubation step bound analytes are labeled by a specific green fluorescent marker. The amount of bound analyte correlates directly the fluorescent intensity allowing the quantication of analytes.

In the Luminex Analyzer the beads are analyzed for their red (analyte classification) and green reporter quantication) fluorescence.

Bead-based assays results in multianalyte profiles that provides an analytic value superior to any single parameter measurement.